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Image Search Results
Journal: bioRxiv
Article Title: Unconventional IFNω -like genes dominate the type I IFN locus and the constitutive antiviral responses in bats
doi: 10.1101/2024.02.22.581518
Figure Lengend Snippet: ( A ) The thermo-stability comparison. The specified IFN proteins (10 -3 µg/ml for each) were incubated at 4 ℃ or 40 ℃ for 4 hours, followed by incubation with RfKT cells at 37℃ for 6 hours. After a 24 hour infection, the expression levels of viral genes were analyzed using qPCR. The decreasing or increasing of V-RNA was shown as arrows. ( B ) Detection of the anti-proliferative activity of IFNs. Cell proliferation was measured by Cell Counting Kit-8 (CCK8) or BrdU assay measuring newly synthesized DNA in cells (0, 24, 48 or 72 h for CCK8, 48 h for BrdU). All experiments were repeated three times with similar results and a representative result is shown. One-way ANOVA with Dunnett’s correction was used for statistics (*P < 0.05; ***P < 0.001; ns no significance).
Article Snippet: BrdU that inserted to
Techniques: Comparison, Incubation, Infection, Expressing, Activity Assay, Cell Counting, BrdU Staining, Synthesized